Microarray & Genomic Analysis Shared Res

Brian Dalley, Microarray Core Director

Summary

Microarray Core Facility

Brian Dalley, Director brian.dalley@hci.utah.edu
585-7192 (office)
581-6346 (lab)
3363 Huntsman Cancer Institute

Summary

The Microarray Core Facility provides researchers at the University of Utah with access to the Affymetrix and Agilent Technologies microarray platforms. These services are complimented by the recent acquisition of an Illumina Genome Analyzer which enables the shared resource to also perform massively parallel DNA sequencing. The combined support for these technologies offers a diverse set of tools for researchers that include measuring gene, exon, or miRNA expression, SNP and DNA copy number profiling, location analysis of DNA binding proteins, defining DNA methylation status, and the sequencing of genomic DNA or resequencing of targeted regions of DNA.

The Microarray Core Facility is operated as a full service facility and is therefore able to provide experimental handling of all aspects of the Agilent and Affymetrix microarray and of the Illumina sequencing processes. These services include evaluation of nucleic acid quality, labeling of samples with modified nucleotides (cy dyes or biotin), microarray hybridization, microarray scanning, feature extraction and annotation of scanned images, Illumina sequencing library preparation, Illumina cluster generation and sequencing of prepared libraries on an Illumina Genome Analyzer. Additional analysis of data generated by the Microarray Shared Resource is accomplished through the Bioinformatics Facility.

The facility is housed in the Huntsman Cancer Institute Building and consists of 800 ft² of lab space, including a class 100 clean room for microarray scanning. Instrumentation at the facility includes a NanoDrop ND-1000 Spectrophotometer, an Agilent Bioanalyzer, an Agilent Microarray Scanner, two Agilent hybridization ovens, an Affymetrix GeneChip Hybridization Oven 640, an Affymetrix Fluidics Station 450, and an Affymetrix workstation, two Bio-Rad DNA Engine thermal cyclers, an Applied Biosystems GeneAmp pcr System 9700, an Illumina Cluster Station, and an Illumina Genome Analyzer II.

Members	Phone	Email
Brian Dalley	801-585-7192	brian.dalley@hci.utah.edu
Kornelia Edes	801-581-6633	kornelia.edes@hci.utah.edu
Nicole Moss	801-581-6346	nicole.moss@hci.utah.edu
Qian Yang	801-581-6346	qian.yang@hci.utah.edu