RESEARCH AND PROFESSIONAL EXPERIENCE:
Education
June 1987 — B.S. Lewis and Clark College
Chemistry (Honors)
April 1995 — Ph.D. Stanford University, Cell Biology
Advisor; Roger Kornberg Ph.D., Professor
Positions Held
May 1995 — American Cancer Society Postdoctoral Fellow
May 1996 — Dept. of Structural Biology, Stanford University
Advisor; Roger Kornberg Ph.D., Professor
May 1996 — Leukemia Society of America Postdoctoral Fellow
June 1998 — Dept. of Genetics, Harvard Medical School
Advisor; Fred Winston Ph.D., Professor
June 1998 — Huntsman Cancer Institute, University of Utah School of Medicine
Assistant Professor, Department of Oncological Science
July 2000 — Assistant Investigator, Howard Hughes Medical Institute
Publications
Cairns, B.R., Collard, M.L. and Landfear, S.M. A developmentally-regulated
gene from Leishmania encodes a putative membrane transport
protein. Proc. Natl. Acad. Sci. USA 86, 7682-7686
(1989)
Stein, D.S., Cairns, B.R. and Landfear, S.M. A developmentally-regulated
transporter in Leishmania
is encoded by a family of clustered genes. Nulc.
Acids Res. 18, 1549-1547 (1990)
Genske, J.E., Cairns, B.C., Stack, S.P. and Landfear, S.M. Structure
and regulation of histone H2B mRNA’s from Leishmania entriettii. Mol.
Cell.Biol. 11, 240-249 (1991)
Cairns, B.R., Ramer, S.W. and Kornberg, R.D. Order of action of
components in the mating pathway revealed with a dominant allele
of the STE11 kinase and the multiple phosphorylation of the STE7
kinase. Genes and Dev. 6, 1305-1318 (1992)
Cairns, B.R., Kim, Y.J., Sayre, M.H., Laurent, B.C. and Kornberg,
R.D. A multisubunit complex containing the SWI1/ADR6, SWI2/SNF2,
SWI3, SNF5 and SNF6 gene products isolated from yeast. Proc.
Natl. Acad. Sci. USA 91, 1950-1954 (1994)
Treich, I., Cairns, B.R., Santos, T., Brewster, E. and Carlson,
M. SNF11, a new component of the yeast SNF-SWI complex that interacts
with a conserved region of SNF2. Mol. Cell Biol. 15, 4240-4248 (1995)
Cairns, B.R., Henry, L.H. and Kornberg, R.D. TFG3/TAF30/ANCI, a
component of the yeast SWI/SNF complex that is similar to the leukemogenic
proteins ENL and AF-9. Mol. Cell.
Biol. 16, 3308-3316 (1996)
Bardwell, L., Cook, J.G., Chang, E.C., Cairns, B.R. and Thorner,
J. Signaling in the yeast pheromone response pathway: specific
and high-affinity interaction of the mitogen-activated protein (MAP)
kinase Kss1 and Fus3 with the upstream MAP kinase STE7. Mol.
Cell Biol. 16, 3637-3650 (1996)
Wang, W., Yutong, X., Zhou, S., Kuo, A., Cairns, B.R. and Crabtree,
G.R. Diversity and specialization of mammalian SWI/SNF complexes.
Genes and Dev. 10,
2117-2130 (1996)
Cairns, B.R., Levinson, R.L., Yamamoto, K.R. and Kornberg, R.D.
Essential role of Swp73p in the function of yeast SWI/SNF complex.
Genes and Dev. 10,
2131-2144 (1996)
Miller, M.E., Cairns, B.R., Levinson, R.L., Yamamoto, K.R., Engel,
D.A. and Smith, M.M. Adenovirus E1A Blocks SWI/SNF-dependent transcriptional
activation. Mol. Cell. Biol. 16, 5737-5743 (1996)
Cairns, B.R., Lorch, Y., Li, Y., Lacomis, L., Erdjument-Bromage,
H., Tempst, P., Du, J., Laurent, B. and Kornberg, R.D. RSC, an
abundant, essential chromatin-remodeling complex. Cell
87, 1249-1260 (1996)
Cao, Y., Cairns, B.R., Kornberg, R.D. and Laurent, B.C. Sfh 1p,
a component of a novel chromatin remodeling complex, is required
for cell cycle progression. Mol. Cell Biol. 17, 3323-3334 (1997)
Cairns, B.R. Chromatin remodeling machines similar motors, ulterior
motives. TIBS. 23, 20-25 (1998)
Lorch, Y., Cairns, B.R., Zheng, M. and Kornberg, R.D. Activated
RSC-nucleosome complex. Cell 94, 29-34 (1998)
Cairns, B.R., Tempst, P., Winston, F. and Kornberg, R.D. Actin-related
proteins are shared functional components of the chromatin remodeling
complexes RSC and SWI/SNF. Molecular Cell 2, 639-651 (1998)
Whitehouse, I., Flaus, A., Cairns, B.R., White, M.F., Workman, J.,
and Owen-Hughes, T. Catalytic nucleosome mobilisation mediated by
the SWI/SNF complex. Nature 400, 784-787 (1999)
Neely, K.E., Hassan, A.H., Wallberg, A.E., Steger, D.J., Cairns,
B.R., Wright, A.P., and Workman, J. Activation domain-mediated
targeting of the SWI/SNF complex to promoters stimulates transcription
from nucleosome arrays. Molecular Cell 2, 649-655 (1999)
Cairns, B.R., Schlichter, A., Tempst, P., Kornberg, R. D., and Winston,
F. Rsc1 and Rsc2 are in distinct forms of RSC and contain AT-hook,
BAH, and bromodomains essential for the function of each complex.
Molecular Cell 2,
715-723 (1999)
S.M. Sengupta, M. VanKanegan, J. Persinger, C. Logie, B.R. Cairns,
C.L. Peterson, and B. Bartholomew The interactions of yeast SWI/SNF
and RSC with the nucleosome before and after chromatin remodeling.
J. Biol. Chem. 10, 1074-1082 (2001)
Angus-Hill, M.L., Schlichter, A., Roberts, D., Erdjument-Bromage,
H., Tempst, P., and Cairns, B.R. (2001) A Rsc3-Rsc30 Zinc Cluster
Heterodimer Reveals New Roles for the Chromatin Remodeling Complex
RSC in Gene Expression and Cell Cycle Control. Molecular Cell (in press).
Szerlong H, Saha A, Cairns BR.
The nuclear actin-related proteins Arp7 and Arp9: a dimeric module
that cooperates with architectural proteins for chromatin remodeling.
EMBO J. 2003 Jun 16;22(12):3175-87.
Douglas N. Roberts, Allen J. Stewart, Jason T. Huff, and Bradley
R. Cairns
The RNA polymerase III transcriptome revealed by genome-wide localization
and activity-occupancy relationships.
Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14695-700
Margaret Kasten, Heather Szerlong, Hediye Erdjument-Bromage, Paul
Tempst, Michel Werner, and Bradley R. Cairns
Tandem bromodomains in the chromatin remodeler RSC recognize acetylated
histone H3 Lys 14.
EMBO J. 2004 (23) 1348-1359
Zhang H, Richardson DO, Roberts DN, Utley R, Erdjument-Bromage H,
Tempst P, Cote J, Cairns BR
The Yaf9 component of the SWR1 and NuA4 complexes is required for
proper gene expression, histone H4 acetylation, and Htz1 replacement
near telomeres
Mol Cell Biol. 2004 Nov;24(21):9424-36
Schlichter, A., and Cairns, B.R. (2005) Histone trimethylation by Set1 is coordinated by the RRM, autoinhibitory and catalytic domains. EMBO Journal, 24, 1222-1231.
Chai, B., Huang, J., Cairns, B.R. and Laurent, B.C. (2004) Distinct roles for the RSC and Swi/Snf ATP-dependent chromatin remodelers in DNA double-stranded break repair. Genes Dev. 19(14):1656-61.
Saha, A., Wittmeyer, J., and Cairns, B.R. (2005) Chromatin remodeling through DNA translocation from an internal nucleosomal site. Nature Structural & Mol. Biol., 12(9):747-55.
Wilson, B., Erdjument Bromage, H., Tempst, P., and Cairns B.R. The RSC chromatin remodeling complex bears an essential yeast-specific module with broad functional roles. Genetics (in press)
Zhang, H.,
Roberts, D., and Cairns, B.R. (2005) Genome-wide dynamics
of Htz1, a conserved histone H2A variant that poises repressed genes for
activation through histone loss. Cell
Guoping Da,
Jeffrey Lenkart, Kehao Zhao, Ramin Shiekattar, Bradley R. Cairns, and Ronen
Marmorstein. (2006) Structure
and function of the SWIRM domain, a conserved protein module found in chromatin
regulatory complexes. PNAS 103(7):2057-2062.
Supplemental method and images for above
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