Investigator, HHMI
Professor, Dept. of Oncological Sciences
John & Karen Huntsman Presidential
Professor in Cancer Research

Huntsman Cancer Institute
University of Utah
2000 Circle of Hope
Salt Lake City, Utah 84112-0555

Phone: 801-585-1822
Lab Phone: 801-585-1823
Asst Phone: 801-587-4749

Email: brad.cairns@hci.utah.edu

RESEARCH AND PROFESSIONAL EXPERIENCE:

Education

Positions Held

Publications

Hammoud, S., Nix, D., Zhang, H., Purwar, J., Carrell, D., Cairns, B. Distinctive chromatin in human sperm packages genes for embryo development (2009) Nature, [epub ahead of print]

Cedric R. Clapier and B.R. Cairns (2009) The Biology of Chromatin Remodeling Complexes. Annual Review of Biochemistry Vol. 78: 273-304 [epub ahead of print]

Rai K, Huggins IJ, James SR, Karpf AR, Jones DA, Cairns BR. DNA demethylation in zebrafish involves the coupling of a deaminase, a glycosylase, and gadd45 Cell 2008 Dec 26; 13 (7):1201-12. Data

Dutrow, N., Nix, D., Holt, D., Milash, B., Dalley, B., Westbroek, E., Parnell, T., and Cairns, B.R. Dynamic Transcriptome of Schizosaccharomyces pombe shown by RNA-DNA hybrid mapping. Nature Genetics 2008; 40(8), 977-986.

Szerlong, H., Hinata, K., Viswanathan, R., Erdjument-Bromage, H., Tempst, P., and Cairns B.R. The HSA domain binds actin-related proteins to regulate chromatin remodeling ATPases. Nature Str. & Mol. Biol. 2008; 15(5), 469-476.

Parnell, T., Huff, J.T., and Cairns, B.R. (2007) RSC regulates nucleosome positioning at Pol II genes and density at Pol III genes. EMBO Journal (Dec. 6, 2007 Epub)

Fischer, C. Saha, A., and Cairns B. R. (2007) Kinetic Model for the ATP-dependent Translocation of S. cerevisiae RSC along double-stranded DNA. Biochemistry 46(43):12416-26.

VanDemark, A.P., Kasten M.M., Ferris, E., Heroux, A., Hill, C.P., and Cairns B.R. (2007) Autoregulation of the Rsc4 Tandem Bromodomain by Gcn5 Acetylation. Molecular Cell, 27(5):817-28.

Gordon, M., Holt, D., Panigrahi, A., Wilhelm, B.T., Erdjument-Bromage, H., Tempst, P., Bähler, J., and Cairns B. R. (2007) Genome-wide dynamics of SAPHIRE, an essential complex for gene activation and chromatin boundaries. Molecular and Cellular Biology 27(11), 4058-69.

Leschziner A.E., Saha, A. , Wittmeyer, J., Zhang, Y., Bustamante, C., Cairns, B.R., and Nogales, E. (2007) Conformational flexibility in the chromatin remodeler RSC observed by electron microscopy and the orthogonal tilt reconstruction method. PNAS 104(12):4913-8.

Rai, K., S., Chidester, S., Svala C.V., Manos, E.J., James, S.R., Karpf, A.R., Jones, D.A. and Cairns, B.R. (2007) Dnmt2 functions in the cytoplasm to promote liver, brain, and retina development in zebrafish. Genes & Dev. 21(3), 261-266.

Zhang Y. , Smith, C.L. , Saha, A., Grill, S.W. , Mihardja, S., Smith S. B. , Cairns B. R., Peterson C. L. , and Bustamante C. (2006) DNA Translocation and Loop Formation Mechanism of Chromatin Remodeling by SWI/SNF and RSC. Molecular Cell 24, 559-568.

Rai, K., Nadauld, L.D., Chidester S., Manos, E.J., James S.R., Karpf, A.R., Cairns, B.R., and Jones, D.A. (2006) Zebrafish dnmt1 and suv39h1 have overlapping and organ-specific functions in zebrafish development. Molecular and Cellular Biology, 26; 7077-7085

Shi X, Hong T, Walter KL, Ewalt M, Michishita E, Hung T, Carney D, Pena P, Lan F, Kaadige MR, Lacoste N, Cayrou C, Davrazou F, Saha A, Cairns BR, Ayer DE, Kutateladze TG, Shi Y, Cote J, Chua KF, and Gozani O. (2006) ING2 PHD domain links histone H3 lysine 4 methylation to active gene repression. Nature. Jul 6;442(7098):96-9.

Bordas-Le Floch, V., Gendrel, G., Soutorina, J., Flores, A, Dumay-Odelot H., Soularue, P., Navarro, F., Cairns, B.R., Lefebvre, O., and Werner, M. (2006) The Rsc4 C-terminus connects the chromatin remodeler RSC to RNA polymerases. Molecular and Cellular Biology, 26 (13), 4920-4933.

Roberts, D., Wilson, B., Huff, J., Stewart, A., and Cairns, B.R. (2006) Dephosphorylation and genome-wide association of Maf1 with Pol III genes in transcriptional repression. Molecular Cell, 22 (5) 633-644.

Da, G., Lenkart, J., Zhao, K., Shiekhattar, R., Cairns, B. R. and Marmorstein, R. (2006) Structure and function of the SWIRM domain, a conserved protein module found in chromatin regulatory complexes. Proc. Natl. Acad. Sci., 103 (7), 2057-2063.

Wilson, B., Erdjument Bromage, H., Tempst, P., and Cairns B.R. (2006) The RSC chromatin remodeling complex bears an essential yeast-specific module with broad functional roles. Genetics, 172 (2), 795-809.

Guoping Da, Jeffrey Lenkart, Kehao Zhao, Ramin Shiekattar, Bradley R. Cairns, and Ronen Marmorstein. (2006) Structure and function of the SWIRM domain, a conserved protein module found in chromatin regulatory complexes. PNAS 103(7):2057-2062. Supplemental method and images for above Table 3 Table 4

Zhang, H., Roberts, D., and Cairns, B.R. (2005) Genome-wide dynamics of Htz1, a conserved histone H2A variant that poises repressed genes for activation through histone loss. Cell

Wilson, B., Erdjument Bromage, H., Tempst, P., and Cairns B.R. The RSC chromatin remodeling complex bears an essential yeast-specific module with broad functional roles.  Genetics (in press)

Saha, A., Wittmeyer, J., and Cairns, B.R. (2005) Chromatin remodeling through DNA translocation from an internal nucleosomal site. Nature Structural & Mol. Biol., 12(9):747-55.

Schlichter, A., and Cairns, B.R. (2005) Histone trimethylation by Set1 is coordinated by the RRM, autoinhibitory and catalytic domains. EMBO Journal, 24, 1222-1231.

Chai, B., Huang, J., Cairns, B.R. and Laurent, B.C. (2004) Distinct roles for the RSC and Swi/Snf ATP-dependent chromatin remodelers in DNA double-stranded break repair. Genes Dev. 19(14):1656-61.

Zhang H, Richardson DO, Roberts DN, Utley R, Erdjument-Bromage H, Tempst P, Cote J, Cairns BR
The Yaf9 component of the SWR1 and NuA4 complexes is required for proper gene expression, histone H4 acetylation, and Htz1 replacement near telomeres
Mol Cell Biol. 2004 Nov;24(21):9424-36

Margaret Kasten, Heather Szerlong, Hediye Erdjument-Bromage, Paul Tempst, Michel Werner, and Bradley R. Cairns
Tandem bromodomains in the chromatin remodeler RSC recognize acetylated histone H3 Lys 14.
EMBO J. 2004 (23) 1348-1359

Douglas N. Roberts, Allen J. Stewart, Jason T. Huff, and Bradley R. Cairns
The RNA polymerase III transcriptome revealed by genome-wide localization and activity-occupancy relationships.
Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14695-700

Szerlong H, Saha A, Cairns BR.
The nuclear actin-related proteins Arp7 and Arp9: a dimeric module that cooperates with architectural proteins for chromatin remodeling.
EMBO J. 2003 Jun 16;22(12):3175-87.

Angus-Hill, M.L., Schlichter, A., Roberts, D., Erdjument-Bromage, H., Tempst, P., and Cairns, B.R. (2001) A Rsc3-Rsc30 Zinc Cluster Heterodimer Reveals New Roles for the Chromatin Remodeling Complex RSC in Gene Expression and Cell Cycle Control. Molecular Cell (in press).

S.M. Sengupta, M. VanKanegan, J. Persinger, C. Logie, B.R. Cairns, C.L. Peterson, and B. Bartholomew The interactions of yeast SWI/SNF and RSC with the nucleosome before and after chromatin remodeling. J. Biol. Chem. 10, 1074-1082 (2001)

Cairns, B.R., Schlichter, A., Tempst, P., Kornberg, R. D., and Winston, F. Rsc1 and Rsc2 are in distinct forms of RSC and contain AT-hook, BAH, and bromodomains essential for the function of each complex. Molecular Cell 2, 715-723 (1999)

Neely, K.E., Hassan, A.H., Wallberg, A.E., Steger, D.J., Cairns, B.R., Wright, A.P., and Workman, J. Activation domain-mediated targeting of the SWI/SNF complex to promoters stimulates transcription from nucleosome arrays. Molecular Cell 2, 649-655 (1999)

Whitehouse, I., Flaus, A., Cairns, B.R., White, M.F., Workman, J., and Owen-Hughes, T. Catalytic nucleosome mobilisation mediated by the SWI/SNF complex. Nature 400, 784-787 (1999)

Cairns, B.R., Tempst, P., Winston, F. and Kornberg, R.D. Actin-related proteins are shared functional components of the chromatin remodeling complexes RSC and SWI/SNF. Molecular Cell 2, 639-651 (1998)

Lorch, Y., Cairns, B.R., Zheng, M. and Kornberg, R.D. Activated RSC-nucleosome complex. Cell 94, 29-34 (1998)

Cairns, B.R. Chromatin remodeling machines ­ similar motors, ulterior motives. TIBS. 23, 20-25 (1998)

Cao, Y., Cairns, B.R., Kornberg, R.D. and Laurent, B.C. Sfh 1p, a component of a novel chromatin remodeling complex, is required for cell cycle progression. Mol. Cell Biol. 17, 3323-3334 (1997)

Cairns, B.R., Lorch, Y., Li, Y., Lacomis, L., Erdjument-Bromage, H., Tempst, P., Du, J., Laurent, B. and Kornberg, R.D. RSC, an abundant, essential chromatin-remodeling complex. Cell 87, 1249-1260 (1996)

Miller, M.E., Cairns, B.R., Levinson, R.L., Yamamoto, K.R., Engel, D.A. and Smith, M.M. Adenovirus E1A Blocks SWI/SNF-dependent transcriptional activation. Mol. Cell. Biol. 16, 5737-5743 (1996)

Cairns, B.R., Levinson, R.L., Yamamoto, K.R. and Kornberg, R.D. Essential role of Swp73p in the function of yeast SWI/SNF complex. Genes and Dev. 10, 2131-2144 (1996)

Wang, W., Yutong, X., Zhou, S., Kuo, A., Cairns, B.R. and Crabtree, G.R. Diversity and specialization of mammalian SWI/SNF complexes. Genes and Dev. 10, 2117-2130 (1996)

Bardwell, L., Cook, J.G., Chang, E.C., Cairns, B.R. and Thorner, J. Signaling in the yeast pheromone response pathway: specific and high-affinity interaction of the mitogen-activated protein (MAP) kinase Kss1 and Fus3 with the upstream MAP kinase STE7. Mol. Cell Biol. 16, 3637-3650 (1996)

Cairns, B.R., Henry, L.H. and Kornberg, R.D. TFG3/TAF30/ANCI, a component of the yeast SWI/SNF complex that is similar to the leukemogenic proteins ENL and AF-9. Mol. Cell. Biol. 16, 3308-3316 (1996)

Treich, I., Cairns, B.R., Santos, T., Brewster, E. and Carlson, M. SNF11, a new component of the yeast SNF-SWI complex that interacts with a conserved region of SNF2. Mol. Cell Biol. 15, 4240-4248 (1995)

Cairns, B.R., Kim, Y.J., Sayre, M.H., Laurent, B.C. and Kornberg, R.D. A multisubunit complex containing the SWI1/ADR6, SWI2/SNF2, SWI3, SNF5 and SNF6 gene products isolated from yeast. Proc. Natl. Acad. Sci. USA 91, 1950-1954 (1994)

Cairns, B.R., Ramer, S.W. and Kornberg, R.D. Order of action of components in the mating pathway revealed with a dominant allele of the STE11 kinase and the multiple phosphorylation of the STE7 kinase. Genes and Dev. 6, 1305-1318 (1992)

Genske, J.E., Cairns, B.C., Stack, S.P. and Landfear, S.M. Structure and regulation of histone H2B mRNA’s from Leishmania entriettii. Mol. Cell.Biol. 11, 240-249 (1991)

Stein, D.S., Cairns, B.R. and Landfear, S.M. A developmentally-regulated transporter in Leishmania is encoded by a family of clustered genes. Nulc. Acids Res. 18, 1549-1547 (1990)

Cairns, B.R., Collard, M.L. and Landfear, S.M. A developmentally-regulated gene from Leishmania encodes a putative membrane transport protein. Proc. Natl. Acad. Sci. USA 86, 7682-7686 (1989)

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