We have gone on to study the effects of depleting Nup153 in somatic cells (HeLa in this case). This led to several interesting observations and is described in Mackay et al., 2009. In addition to defects early in mitosis, one striking phenotype was a delay in the final stages of cell division. We tracked this phenotype both with live cell imaging (as further described in a video protocol, Mackay et al, 2010a) and by monitoring midbodies, which are structures formed between the two daughter cells that organize the final separation of the cells, or abscission. This unexpected finding led us to a new interest in how the role of Nup153 is tied into midbodies, and we found that defects in assembly of the nuclear pore basket trigger an Aurora B mediated abscission checkpoint (Mackay et al., 2010b, Mackay and Ullman, 2011). We are now interested in elucidating the molecular link that connects defects in basket architecture to Aurora B.